Authur荧光细胞计数分析仪

国产进口 : 进口
产地品牌 : 韩国/NanoEntek
型号 : Arthur

总访问 : 2577
产品类别 : 细胞计数
最后更新 : 2026-02-05 16:22:25

产品介绍

公司简介

Authur荧光细胞分析仪是是集双荧光通道的显微镜功能、图像分析和细胞计数于一体的仪器。这台自动图像采集分析设备为细胞计数，细胞活率以及细胞凋亡和细胞周期检测提供一体化解决方案。

Authur荧光细胞分析仪特点：

• 高精度

• 可以获得精确地，全面的实验结果

• 可以获得细胞图片和细胞计数

• 筛选细胞

• 多功能多通道

• 适用广泛的真核细胞

• 也适用于悬浮细胞的各种实验

• 快速细胞计数及分析

• 1分钟内3通道的细胞计数

• 只需要25ul样本

• 使用简便

• 无需校正，一体机触摸屏操作

Authur荧光细胞分析仪应用

Cell viability assay

Cell line: Jurkat

Figure 2. Comparison of GFP expression profile in two platforms

U-2 OS cells were transfected using the Neon™ transfection system (from Life Technologies) and 0.25 µg of the five different GFP constructs and then analyzed on the Arthur™ and competitor’s product F to determine transfection efficiency. The histograms in panels A (Arthur™) and C (competitor’s product F) show the green fluorescence intensities and counts for the transfected populations. The percentages of GFP-expressing cells detected with the Arthur™ were consistent with the results from the competitor’s product F. ‘Colored circles’ option makes it easy to identify gated cells (Panel B); colored circles represent: cells expressing GFP (green circles), non-GFP-expressing cells (blue circles). The image in panel D shows the GFP expressing cells before trypsinization under the conventional fluorescence microscope.

GFP expression assay

GFP-fusion construct 1		GFP-fusion construct 2

Annexin V apoptosis analysis

Figure 3. Arthur™ allows confirmation of apoptosis assay using the visual display of the samples

Following 18 hour-treatment of Staurosporine, HeLa cells were stained with apoptosis kit and subjected to Arthur™ and competitor’s product F. The apoptosis kit contains an annexin V–Alexa Fluor® 488 conjugate and PI to differentiate live (annexin V–negative/PI-negative), dead (PI-positive), and apoptotic (annexin V–positive/PI-negative) cells. Using Arthur™, 20 % of the cell population were measured as live, 38 % apoptotic, and 42 % dead, confirming that the Arthur™ provides quantitative data comparable to those collected from competitor’s product F.

Cell cycle assay

Figure 4. Comparison of cell cycle profiles in two platforms:
Arthur™ and Competitor’s product F

HeLa cells were treated with Nocodazole (microtubule-destabilizer) or Epothilone B
(microtubule-stabilizer) to arrest cells in G2/M phase. Cells were fixed with 70%
ethanol and stained with Propidium Iodide(PI). The histograms show the percentages
of G0/G1 phase, S phase and G2/M phase of cell cycle analysis for drug treated
HeLa cells on the Arthur™ (A) and Competitor’s product F (B).
The cell cycle analysis of Arthur™ was highly consistent with the results obtained
from competitor’s product F.

Specifications

Counting time	10 sec. ~ 2 min.
Cell measurement range	1 × 10⁵ - 1 × 10⁷ cells/mL
Cell size range	5 - 60 m
Sample volume	25 µL
Optics	3 channels (bright field, green fluorescence and red fluorescence)
Excitation	Green channel LED: 458 ± 20 nm Red channel LED: 530 ± 20 nm
Filters	Green channel: 466/40 EX, 495 LP Di, 525/50 EM Red channel: 543/22 EX, 580 LP Di, 585 LP EM
Camera	1.3 Mega pixels, 4× objective, 4× or 16× digital zoom
USB drive	4 GB
Operating power	100-240 V, 2.5 A, 120 W
Frequency	50 / 60 Hz
Dimension	290 mm (W) × 440 mm (D) × 290 mm (H)
Weight	8.7 kg (19.4 lbs)
Operating environment	5 - 40 °C, 20 - 95 %

Ordering information

Cat. No.	Description	Contents
AT1000	Arthur Novel fluorescence cell counter	Main device, Power supply, USB drive, ArthurTM Cell analysis slide (50 slides)
AC0050	ArthurTM Cell analysis slide	50 slides (100 counts)
AC0500	ArthurTM Cell analysis slide	500 slides (1,000 counts)
AB0100	ArthurTM Calibration beads	Alignment beads, Green calibration beads, Red calibration beads (100 μL/tube)